Acute diarrhoea due to a Shiga toxin 2e-producing Escherichia coli O8 : H19 Saupe, Angela and Edel, Birgit and Pfister, Wolfgang and Löffler, Bettina and Ehricht, Ralf and Rödel, Jürgen,, 4, e005099 (2017), doi = https://doi.org/10.1099/jmmcr.0.005099, publicationName = Microbiology Society, issn = , abstract= Introduction. Identification of non-O157 Shiga-toxin-producing Escherichia coli (STEC) infections may be underestimated in microbiological diagnosis. Case presentation. A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for stx2 . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic E. coli media. Molecular characterization was performed and identified the isolate as stx 2e-positive STEC of serotype O8 : H19. The strain harboured lpfA, but not eae. Conclusion. This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection., language=, type=