1887

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Volume 73, Issue 3

and assessment of anti-biofilm and anti-quorum sensing properties of 2,4-Di-tert butylphenol against No Access

Abstract

is a nosocomial pathogen with a high potential to cause food-borne infections. It is designated as a critical pathogen by the World Health Organization due to its multi-drug resistance and mortalities reported. Biofilm governs major virulence factors, which promotes drug resistance in . Thus, a compound with minimum selection pressure on the pathogen can be helpful to breach biofilm-related virulence.

To identify anti-biofilm and anti-virulent metabolites from extracts of wild (mango) brine pickle bacteria that diminishes pathogenesis and resistance of .

This study reports anti-biofilm and anti-quorum sensing (QS) efficacy of secondary metabolites from bacterial isolates of fermented food origin.

Cell-free supernatants (CFS) of 13 bacterial isolates from fermented mango brine pickles were screened for their efficiency in inhibiting biofilm formation and GC-MS was used to identify its metabolites. Anti-biofilm metabolite was tested on early and mature biofilms, pellicle formation, extra polymeric substances (EPS), cellular adherence, motility and resistance of . Gene expression and studies were also carried out to validate the compounds efficacy.

CFS of TMP6b identified as inhibited biofilm production (83.02 %). Of these, major compound was identified as 2,4-Di--butyl phenol (2,4-DBP). At sub-lethal concentrations, 2,4-DBP disrupted both early and mature biofilm formation. Treatment with 2,4-DBP destructed biofilm formed on glass and plastic. In addition, key virulence traits like pellicle (77.5 %), surfactant (95.3 %), EPS production (3-fold) and cell adherence (65.55 %) reduced significantly. cells treated with 2,4-DBP showed enhanced sensitivity towards antibiotics, oxide radicals and blood cells. Expression of biofilm-concomitant virulence genes like , , , , , and along with QS genes significantly decreased. The studies further validated the higher binding affinity of 2,4-DBP to the AbaR protein than the cognate ligand molecule.

To our knowledge, this is the first report to demonstrate 2,4- DBP has anti-pathogenic potential alone and with antibiotics by and studies against . It also indicates its potential use in therapeutics and bio-preservatives.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 2,4-Di-tert-butyl phenol , Acinetobacter baumannii , anti-biofilm , anti-quorum sensing and exopolymeric substances
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2024-03-20
2024-04-27
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In vitro and in silico assessment of anti-biofilm and anti-quorum sensing properties of 2,4-Di-tert butylphenol against Acinetobacter baumannii
J. Med. Microbiol. 73, 001813 (2024); https://doi.org/10.1099/jmm.0.001813
/content/journal/jmm/10.1099/jmm.0.001813
/content/journal/jmm/10.1099/jmm.0.001813
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