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Volume 74, Issue 4

sp. nov., a bacterium possessing metallophore activity towards rare earth elements No Access

Abstract

A polyphasic taxonomic study was carried out on strain ES2, isolated from sediment of a wetland created to remediate acid drainage from a coal mine. The rod-shaped bacterium formed yellow/orange pigmented colonies and produced the pigment flexirubin. The 16S rRNA gene sequence results assigned the strain to , with 98.9 and 98.3 % similarity to and , respectively. Computation of the average nucleotide identity and digital DNA–DNA hybridization values with the closest phylogenetic neighbours of ES2 revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. The dominant fatty acids of strain ES2 were iso-C, iso-C ω9, iso C 3-OH, and iso-C 2-OH. The DNA G+C content was 35.5 mol%. The major polar lipid was phosphatidylethanolamine while menaquinone-6 was the only menaquinone found. This bacterium has been previously shown to possess metallophore activity towards rare earth elements, and based on genome sequencing, possesses all required genes for siderophore production/activity, possibly identifying the source of this unique ability. On the basis of the results obtained here, this bacterium is assigned to the genus as representing a new species with the name sp. nov., type strain ES2 (=NRRL B-65679=KCTC 102120).

  • Received:
  • Accepted:
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Keyword(s): acid mine drainage , Chryseobacterium , Chryseobacterium metallicongregator , metal(loid) oxyanion , metal(loid) resistance and rare earth elements
Funding
This study was supported by the:
  • Slippery Rock University Faculty Student Research Grant
    • Principle Award Recipient: ChrisMaltman
  • Department of Biology, Slippery Rock University
    • Principle Award Recipient: ChrisMaltman
© 2024 The Authors
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2024-04-30
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Chryseobacterium metallicongregator, sp. nov., a bacterium possessing metallophore activity towards rare earth elements
Int J Syst Evol Microbiol 74, 006337 (2024); https://doi.org/10.1099/ijsem.0.006337
/content/journal/ijsem/10.1099/ijsem.0.006337
/content/journal/ijsem/10.1099/ijsem.0.006337
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