1887

JMM Case Reports

Volume 2, Issue 3

Genome sequence and analysis of resistance and virulence determinants in a strain of causing native-valve endocarditis Open Access

Abstract

Introduction:

colonizes mucosal surfaces in humans and can serve as a reservoir for resistance and virulence determinants for and , but only on rare occasions has this species been documented as a cause of invasive infection. The host and pathogen factors that predispose to invasive infection with this organism are unknown.

Case presentation:

A healthy 27-year-old female presented with fever. was isolated from two sets of blood cultures drawn on arrival. Transoesophageal echocardiogram revealed mitral valve vegetation. The patient was diagnosed with endocarditis by modified Duke's criteria. Genome sequencing was performed on the isolate and comparison was made with previously published resistance and virulence determinants. Although putative virulence factors were present in this isolate, there is not a sufficient database of genetic information for this species to reach conclusions about the role of these virulence factors in predisposing to invasive disease.

Conclusions:

For rare infections, like this case, only through reporting of the clinical course and pathogen genome can a sufficient dataset accumulate to make statistically significant inferences and to motivate hypothesis-based confirmatory experiments. This is the first report of whole genome sequencing for this species to be accompanied by a description of invasive clinical disease, and sets the precedent for future studies.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antimicrobial resistance , DNA-uptake sequence , endocarditis , genome sequence , Neisseria mucosa and virulence factors
© 2015 The Authors
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2015-06-01
2024-04-26
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References

  1. Altschul S.F, Gish W, Miller W, Myers E.W, Lipman D.J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
     [Google Scholar]
  2. Aziz R.K, Bartels D, Best A.A, DeJongh M, Disz T, Edwards R.A, Formsma K, Gerdes S, Glass E.M. other authors 2008; The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9:75 [CrossRef]
     [Google Scholar]
  3. Bennett J.S, Bentley S.D, Vernikos G.S, Quail M.A, Cherevach I, White B, Parkhill J, Maiden M.C. 2010; Independent evolution of the core and accessory gene sets in the genus Neisseria: insights gained from the genome of Neisseria lactamica isolate 020-06. BMC Genomics 11:652 [CrossRef]
     [Google Scholar]
  4. Bennett J.S, Jolley K.A, Earle S.G, Corton C, Bentley S.D, Parkhill J, Maiden M.C. 2012; A genomic approach to bacterial taxonomy: an examination and proposed reclassification of species within the genus Neisseria. Microbiology 158:1570–1580 [CrossRef]
     [Google Scholar]
  5. Bennett J.S, Jolley K.A, Maiden M.C. 2013; Genome sequence analyses show that Neisseriaoralis is the same species as ‘Neisseria mucosa var. heidelbergensis’. Int J Syst Evol Microbiol 63:3920–3926 [CrossRef]
     [Google Scholar]
  6. Bowler L.D, Zhang Q.Y, Riou J.Y, Spratt B.G. 1994; Interspecies recombination between the penA genes of Neisseria meningitidis and commensal Neisseria species during the emergence of penicillin resistance in N. meningitidis: natural events and laboratory simulation. J Bacteriol 176:333–337
     [Google Scholar]
  7. Croucher N.J, Harris S.R, Fraser C, Quail M.A, Burton J, van der Linden M, McGee L, von Gottberg A, Song J.H, other authors. 2011; Rapid pneumococcal evolution in response to clinical interventions. Science 331:430–434 [CrossRef]
     [Google Scholar]
  8. Davidsen T, Rødland E.A, Lagesen K, Seeberg E, Rognes T, Tønjum T. 2004; Biased distribution of DNA uptake sequences towards genome maintenance genes. Nucleic Acids Res 32:1050–1058 [CrossRef]
     [Google Scholar]
  9. Durack D.T, Lukes A.S, Bright D.K. 1994; Duke Endocarditis Service New criteria for diagnosis of infective endocarditis: utilization of specific echocardiographic findings. Am J Med 96:200–209 [CrossRef]
     [Google Scholar]
  10. Gouy M, Guindon S, Gascuel O. 2010; SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 27:221–224 [CrossRef]
     [Google Scholar]
  11. Ingram R.J, Cornere B, Ellis-Pegler R.B. 1992; Endocarditis due to Neisseria mucosa: two case reports and review. Clin Infect Dis 15:321–324 [CrossRef]
     [Google Scholar]
  12. Johnson A.P. 1983; The pathogenic potential of commensal species of Neisseria. J Clin Pathol 36:213–223 [CrossRef]
     [Google Scholar]
  13. Jolley K.A, Maiden M.C. 2010; BIGSdb: Scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics 11:595 [CrossRef]
     [Google Scholar]
  14. Joseph B, Schwarz R.F, Linke B, Blom J, Becker A, Claus H, Goesmann A, Frosch M, Müller T, other authors. 2011; Virulence evolution of the human pathogen Neisseria meningitidis by recombination in the core and accessory genome. PLoS One 6:e18441 [CrossRef]
     [Google Scholar]
  15. Maiden M.C. 2008; Population genomics: diversity and virulence in the Neisseria. Curr Opin Microbiol 11:467–471 [CrossRef]
     [Google Scholar]
  16. Marri P.R, Paniscus M, Weyand N.J, Rendón M.A, Calton C.M, Hernández D.R, Higashi D.L, Sodergren E, Weinstock G.M, other authors. 2010; Genome sequencing reveals widespread virulence gene exchange among human Neisseria species. PLoS One 5:e11835 [CrossRef]
     [Google Scholar]
  17. Pilmis B, Lefort A, Lecuit M, Join-Lambert O, Nassif X, Lortholary O, Charlier C. 2014; Endocarditis due to Neisseria mucosa: case report and review of 21 cases. A rare and severe cause of endocarditis. J Infect 68:601–604 [CrossRef]
     [Google Scholar]
  18. Qvarnstrom Y, Swedberg G. 2006; Variations in gene organization and DNA uptake signal sequence in the folP region between commensal and pathogenic Neisseria species. BMC Microbiol 6:11 [CrossRef]
     [Google Scholar]
  19. Rice P, Longden I, Bleasby A. 2000; EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet 16:276–277 [CrossRef]
     [Google Scholar]
  20. Snyder L.A, McGowan S, Rogers M, Duro E, O'Farrell E, Saunders N.J. 2007; The repertoire of minimal mobile elements in the Neisseria species and evidence that these are involved in horizontal gene transfer in other bacteria. Mol Biol Evol 24:2802–2815 [CrossRef]
     [Google Scholar]
  21. Spratt B.G, Bowler L.D, Zhang Q.Y, Zhou J, Smith J.M. 1992; Role of interspecies transfer of chromosomal genes in the evolution of penicillin resistance in pathogenic and commensal Neisseria species. J Mol Evol 34:115–125 [CrossRef]
     [Google Scholar]
  22. Zerbino D.R, Birney E. 2008; Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18:821–829 [CrossRef]
     [Google Scholar]
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Genome sequence and analysis of resistance and virulence determinants in a strain of Neisseria mucosa causing native-valve endocarditis
JMM Case Rep 2, e000049 (2015); https://doi.org/10.1099/jmmcr.0.000049
/content/journal/jmmcr/10.1099/jmmcr.0.000049
/content/journal/jmmcr/10.1099/jmmcr.0.000049
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